Récent 40+ Acid Pretreatment Of Lignocellulosic Biomass. The objective of this review is to discuss the various pretreatment strategies currently in use and provide an overview of their utilization for the isolation of. Ozone pretreatment is a great option for lignin content reduction in lignocellulosic biomass. Acid pretreatment involves the use of concentrated and diluted acids to break the rigid structure of the lignocellulosic material. Most commonly used acids are sulfuric acid or hydrochloric acid.